b3 addgene cat 58780 (Addgene inc)
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b3 addgene cat 58780
B3 Addgene Cat 58780, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/b3+addgene+58780/pX330S-4+(Plasmid+%2358780)/pmc09217744__41586_2022_4803_MOESM1_ESM-252-45-46
Average 90 stars, based on 6 article reviews
B3 Addgene Cat 58780, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/b3+addgene+58780/pX330S-4+(Plasmid+%2358780)/pmc09217744__41586_2022_4803_MOESM1_ESM-252-45-46
Average 90 stars, based on 6 article reviews
b3 addgene cat 58780 - by Bioz Stars,
2026-10
90/100 stars
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Multiplexing:Article Title: LADL: Light-activated dynamic looping for endogenous gene expression control Article Snippet: Individual gRNA plasmids without soluble CRY2 The Addgene #58768 plasmid was digested with SnaBI and EcoRI to excise the Cas9 open reading frame and ligated with the annealed and phosphorylated dsDNA oligo (MRP173, MRP174; Supplementary Table 1) to get the Ampicillin resistant S12.1 gRNA multiplex backbone (BB) plasmid (Supplementary Figure 2d). .. For Step 1 of multiplexing, single gRNAs were cloned into one of the following plasmids: S12.1 (Ampicillin resistant) (Supplementary Figure 2e), B1 (Addgene # 58778) 21 (Supplementary Figure 2f), B2 (Addgene # 58779) 21 (Supplementary Figure 2g) or Clone Assay:Article Title: LADL: Light-activated dynamic looping for endogenous gene expression control Article Snippet: Individual gRNA plasmids without soluble CRY2 The Addgene #58768 plasmid was digested with SnaBI and EcoRI to excise the Cas9 open reading frame and ligated with the annealed and phosphorylated dsDNA oligo (MRP173, MRP174; Supplementary Table 1) to get the Ampicillin resistant S12.1 gRNA multiplex backbone (BB) plasmid (Supplementary Figure 2d). .. For Step 1 of multiplexing, single gRNAs were cloned into one of the following plasmids: S12.1 (Ampicillin resistant) (Supplementary Figure 2e), B1 (Addgene # 58778) 21 (Supplementary Figure 2f), B2 (Addgene # 58779) 21 (Supplementary Figure 2g) or Plasmid Preparation:Article Title: LADL: Light-activated dynamic looping for endogenous gene expression control Article Snippet: Initially, CRY2 was cloned into S12.1 using the EcoRI and SnaBI sites, and then gRNA 115 was cloned into S12.1. gRNA 117 was cloned into B1 (Addgene # 58778) 21 . .. We multiplexed gRNA 115 plasmid and gRNA 117 plasmid as well as B2 (Addgene # 58779) 21 and Control:Article Title: LADL: Light-activated dynamic looping for endogenous gene expression control Article Snippet: Initially, CRY2 was cloned into S12.1 using the EcoRI and SnaBI sites, and then gRNA 115 was cloned into S12.1. gRNA 117 was cloned into B1 (Addgene # 58778) 21 . .. We multiplexed gRNA 115 plasmid and gRNA 117 plasmid as well as B2 (Addgene # 58779) 21 and |